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Therefore, it also applies in clinical species to find out etiological agents. Tryptic Soy Broth is a general-purpose medium and also applicable for the cultivation of a wide variety of microorganisms.Aspergillus brasiliensis ATCC 16404: luxuriant growth 20 -25 ☌ within 5 days.Candida albicans ATCC 10231: luxuriant growth 20 -25 ☌ within 5 days.Streptococcus pneumoniae ATCC 6305: luxuriant growth 20 -25 ☌ within 3 days.Salmonella Typhimurium ATCC 14028: luxuriant growth 20 -25 ☌ within 3 days.Escherichia coli ATCC 25922: luxuriant growth 20 -25 ☌ within 3 days.Staphylococcus aureus ATCC 25923: luxuriant growth 20 -25 ☌ within 3 days.Sterility Testing- Growth promotion+Validation To say growth nil ( negative): Subculture into solid medium and after incubation check the growth that is without colonies.Aspergillus brasiliensis ATCC 16404 : good-luxuriant.Candida albicans ATCC 10231: good-luxuriant.Staphylococcus aureus ATCC 25923): good-luxuriant.Escherichia coli ATCC 25922: good-luxuriant.

Incubate aerobically at 35-37✬ for 18-24 hours.Inoculate the specimen as soon as possible after collection.Allow the medium to warm at 37☌ or to room temperature.Test procedure ( specimen/organism inoculation) Control strains ( Escherichia coliATCC 25922, Staphylococcus aureusĪTCC 25923, Candida albicans ATCC 10231, Aspergillus brasiliensis ATCC 16404).Test specimens ( samples or growth of test organisms).The product is light and temperature-sensitive protects from light, excessive heat, moisture, and freezing.Media should not be used if there are any signs of deterioration or contamination.Store at 2-8✬ and away from direct light.Storage and Shelf life of Tryptic Soy Broth Store the plates in a refrigerator at 2-8☌.After autoclaving, leave for cooling to room temperature.Sterilize by autoclaving at 15 lbs pressure (121☌) for 15 minutes.Desired volumes and containers ( vial or tube or bottle) can be taken according to the nature of the testing purpose.Heat to boiling to dissolve the medium completely.Suspend3.0 grams in 100 ml purified/distilled or deionized water.Sodium chloride maintains the osmotic balance of the medium. Glucose is the fermentable source of carbon whereas dibasic potassium phosphate serves as the buffer in the medium. Soybean natural sugars promote the growth of fastidious microorganisms.

Papaic digest of soybean meal and pancreatic digest of casein supply amino acids and long-chain peptides for the growth of organisms. PH after sterilization (at 25☌): 7.3☐.2 Principle of Tryptic Soy Broth Composition of Tryptic Soy Brothĭibasic potassium phosphate (K 2HPO 4): 0.25 It is also used in sterility testing of molds and lower bacteria in accordance with United States Pharmacopoeia (USP).

Used in the isolation of: Neisseria meningitides 13090, Staphylococcus epidermidis 12228, Streptococcus pneumoniae 6305, Streptococcus pyogenes 19615, Aspergillus brasiliensis (niger) 16404, Bacillus subtillis 6633, Candida albicans 10231, Escherichia coli 8739, Pseudomonas aeruginosa 9027, Salmonella enterica subsp.Tryptic Soy Broth is a general-purpose medium and uses for the cultivation of a wide variety of microorganisms covering especially common aerobic, facultatively anaerobic bacteria, yeast, and molds.

Because of its capacity for growth promotion, TSB is also recommended for use as the inoculum broth for disc diffusion and agar dilution antimicrobial susceptibility testing as standardized by the Clinical and Laboratory Standards Institute (CLSI).

TSB is recommended for testing bacterial contaminants in cosmetics and complies with established standards in the food industry.

TSB was chosen by the USDA Animal and Plant Health Inspection Service for detecting viable bacteria in live vaccines.

This formulation is included in the USP as a medium for use in performing microbial enumeration tests and tests for specified microorganisms when testing nonsterile pharmaceutical products.

TSB is a nutritious medium that will support the growth of a wide variety of microorganisms, including common aerobic, facultative and anaerobic bacteria and fungi. Tryptic Soy Broth (Soybean-Casein Digest Medium) is a general purpose medium used in qualitative procedures for the cultivation of fastidious and non-fastidious microorganisms from a variety of clinical and nonclinical specimens.